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Participant 9

Danish Technological Institute (DTI)

Partner 9 :
Danish Technological Institute Life Science Division, Food Technology Department Holbergsvej 10, DK 6000 Kolding, Denmark

Key skills:
Functional proteomics, Biomolecular mass spectrometry Laboratory (clinical) background and technical information: The Department of Medical Life Science Division at the Danish Technological Institute (DTI-LIFE) has strong competences within the field of biomolecular mass spectrometry. The Proteomics group at DTI (established in 2001) has several years of expertise on research and collaborations projects with both the industry and academia in the development of advanced mass spectrometry-based methods and strategies for the analysis of large biomolecules including proteins and their post-translational modifications such as glycosylations and phosphorylations. DTI has been involved in a variety of R&D projects spanning from the characterization of antifungal proteins from seeds, identification of stem cell markers to mode-of action/mechanisms of action studies of anti-cancer drugs.

Capacity to contribute to the project:
Partner 9 offers genetic testing such as for alpha-Mannosidosis and has well-equipped analytical laboratory with state-of-the-art equipment covering a variety of standard analysis spanning from routine GMP approved HPLC analysis to advanced mass spectrometry-based biomolecular characterisation. DTI-LIFE will develop methods for the purification, characterization and quantification of selected mannose-oligosaccharides from blood and urine samples collected from groups of healthy persons, untreated and treated patients. Chromatographic methods based on affinity matrices formed from lectins (proteins that binds specific glycan structures) directed against specific mannose-oligosaccharides combined with lysosome isolation or Sep Pack C18 purification will be developed and applied in the purification of mannose-oligosaccharides from blood and urine samples collected from patients in clinical trials. The purified mannose-oligosaccharides will be structurally characterized using nano-LC Orbitrap MSn analysis. Quantitative methods based on HPLC or nano-LC combined with mass spectrometric detection will be developed and applied for the quantification of selected oligosaccharides in samples collected in clinical trials.

Personnel involved:

Key Publications:

  1. Hans Christian Beck (2010). Mass spectrometry in epigenetic research. In “Bioinformatics Methods in Clinical Research” Series: Methods in Molecular Biology , Vol. 593. Matthiesen, Rune (Ed.)
  2. Beck HC, Madsen, SM, Glenting J, Petersen, J, Israelsen H, Nørrelykke MR, Antonssen M, Hansen AM. (2009). Proteomic Analyses of Cell Surface-associated Proteins from probiotic bacterium Lactobacillus plantarum. FEMS Microbiology Letters. 297: 61-66.
  3. Hans Peter Sørensen, Jørgen Petersen, Lone Søvad Bak, Anne Maria Hansen and Hans Christian Beck. (2009). Oat (Avena sativa) seed extracts as an antifungal food preservative through the catalytic activity of a highly abundant class I chitinase. Applied Biochemistry and Biotechnology. Feb 18. [Epub ahead of print]
  4. Morsczeck, C., Petersen, J., Völlner, F., Driemel, O., Reichert, T., and Beck, H.C (2009). Proteomic analysis of osteogenic differentiation of dental follicle precursor cells. Electrophoresis. 30:1175-84.
  5. Beck, Hans C, Eva C. Jensen, Rune Mathiessen, Lars H. Jensen, Paul Finn, Maxwel Sehested, Anne M. Hansen, and Ole N. Jensen (2006). Quantitative proteomic analysis of post-translational modifications of human histones.

 

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